Lab syllabus

Date

LAB EXERCISE

Sept. 2

NO LAB

Sept. 9

• Getting to know each other and our lab space (read lab policies)
• Pipet Olympics (read appendices 1 and 2)
• Learning how to write scientific papers
• Introduction to Blood Cells Lab (read introduction in posted manual)

Sept. 16

• Blood Cells Lab, Part I:  Extract proteins

Sept. 23

• Viewing protein structures in 3D – myoglobin (please download PyMol on your laptop and bring to lab)

Sept. 30

• Blood Cells Lab, Part II:  Protein assays

Oct. 7

• Blood Cells Lab, Part III:  SDS-PAGE analysis
• Introduction to Enzyme Kinetics Lab (read introduction in posted manual)

 (Blood Cells lab report due Wed, 10/21)

Oct. 14

FALL BREAK – NO LAB

Oct. 21

• Enzyme Kinetics Lab part I- Determine the effect of varying enzyme concentration on initial velocity

Oct. 28

Enzyme Kinetics Lab parts II and III

• Enzyme kinetics – Km and Vmax determination
• Inhibitor studies

(Enzyme kinetics lab report due Wed, 11/4)

Nov. 4

• Sugars lab
• Introduction to DHFR lab and induction of DHFR in E. coli (please read introduction in posted manual)

(Sugars lab report due Wed, 11/11)

Nov. 11

• DHFR lab, Part I: Cell lysis and affinity column purification

Nov. 18

• DHFR lab, Part II: Protein assays and specific activity calculation

Nov. 25

DAY BEFORE THANKSGIVING – NO LAB

Dec. 2

• DHFR lab, Part III: SDS-PAGE analysis

(DHFR lab report due Th, 12/10 in lecture) 

Dec. 9

NO LAB – office hours with Mia